Differential quantitative proteomics of Porphyromonas gingivalis by linear ion trap mass spectrometry: Non-label methods comparison, q-values and LOWESS curve fitting
نویسندگان
چکیده
منابع مشابه
Mass Spectrometry-Based Label-Free Quantitative Proteomics
In order to study the differential protein expression in complex biological samples, strategies for rapid, highly reproducible and accurate quantification are necessary. Isotope labeling and fluorescent labeling techniques have been widely used in quantitative proteomics research. However, researchers are increasingly turning to label-free shotgun proteomics techniques for faster, cleaner, and ...
متن کاملQuadrupole ion trap mass spectrometry.
A number of other features of ITMS systems that will enhance their ability to analyze biological macromolecules are worth mentioning. As has already been demonstrated for ESI/quadrupole, ESI/magnetic sector, and ESI/FTICR systems, the capability of inducing fragmentation of the ESI-generated multiply charged ions of biological macromolecules in the capillary/skimmer region of the ESI source and...
متن کاملComparative analysis of statistical methods used for detecting differential expression in label-free mass spectrometry proteomics.
UNLABELLED Label-free LC-MS/MS proteomics has proven itself to be a powerful method for evaluating protein identification and quantification from complex samples. For comparative proteomics, several methods have been used to detect the differential expression of proteins from such data. We have assessed seven methods used across the literature for detecting differential expression from spectral...
متن کاملFOCUS: PROTEOMICS “De Novo” Peptide Sequencing by MALDI- Quadrupole-Ion Trap Mass Spectrometry: A Preliminary Study
Collision-induced dissociation of singly charged peptide ions produced by resonant excitation in a matrix-assisted laser desorption/ionization (MALDI) ion trap mass spectrometer yields relatively low complexity MS/MS spectra that exhibit highly preferential fragmentation, typically occurring adjacent to aspartyl, glutamyl, and prolyl residues. Although these spectra have proven to be of conside...
متن کاملLabeling Methods in Mass Spectrometry Based Quantitative Proteomics
Proteomics is loosely defined as the description of sets of proteins from any biological source, which have in most cases been identified by using mass spectrometry. However, only the mere identity of proteins present in a certain sample does not give any information about the dynamics of the proteome, involving relevant cellular events such as protein synthesis and degradation, or the formatio...
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ژورنال
عنوان ژورنال: International Journal of Mass Spectrometry
سال: 2007
ISSN: 1387-3806
DOI: 10.1016/j.ijms.2006.08.004